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KMID : 1161420190220030248
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Journal of Medicinal Food
2019 Volume.22 No. 3 p.248 ~ p.256
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Cardiovascular Effect of Diosgenin in Ovariectomized Rats
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de Silva Morais Ilmara Cecilia Pinheiro
Moura Iris Jordania Luz
Sabino Carla Kelly Barroso
Nicolau Lucas Antonio Duarte
de Moura Souza Fabiana
da Silva-Filho Jose Couras
de Cassia Meneses Oliveira Rita
Medeiros Jand Venes Rolim
de Lima Sidney Goncalo
de Oliveira Aldeidia Pereira
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Abstract
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Diosgenin is a phytoestrogen and a constituent of Dioscorea. It has several biological effects, and some of them are anti-inflammatory, antidiabetic, antitumor, and vasodilatory. The present study investigated both the vasorelaxing and antioxidant mechanisms of diosgenin in isolated rat aortic rings. Female rats weighing 200?220?g were subjected to sham or OVX operations at 8 weeks of age. Ovariectomy was performed for menopause induction after anesthesia. Diosgenin (10?9 M?3?×?10?4 M) produced a concentration-dependent relaxation in aortic rings precontracted with phenylephrine (1?μM), exhibiting Emax value of 55.34%?±?7.7% (in endothelium-intact rings) and Emax value of 30.30%?±?5.7% (in endothelium-denuded rings). In the endothelium-intact rings, the vasorelaxing effect of diosgenin was reduced by NG-nitro-l-arginine methyl ester (L-NAME) (100?μM), atropine (1?μM), indomethacin (10?μM), 1H-[1,2,4]oxadiazolo-[4,3-a]quinoxalin-1-one (ODQ) (10?μM), 4-aminopyridine (1?mM), tetraethylammonium (3?mM), glibenclamide (10?μM), apamin (10?μM), and Tiron (1?μM). Diosgenin (10?5 M) inhibited the contractions induced by cumulative addition of phenylephrine (10?9?10?5 M). The 28-days treatment with diosgenin (50?mg/kg, v.o.) did not imply changes in the myeloperoxidase parameter, but increased significantly, levels of glutathione, superoxide dismutase, and nitric oxide, as well as reduced the concentration of malondialdehyde related to lipid peroxidation. Our results suggest that diosgenin induced relaxation in aortic rings via an endothelium-dependent pathway, which involves the EDRF, the opening of potassium channels and antioxidant action.
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KEYWORD
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antioxidant effect, endothelium, phytoestrogen
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